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This study was supported by the Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas (Innovative Bioproduction Kobe), MEXT, Japan

After the Matrigel had fully solidified, the unsolidified liquid in the upper chamber was aspirated, and 70 L of culture medium was gently added

Apply once per day and increase to twice a day if well tolerated

This interaction reduces inhibitory binding between caveolin-1 and endothelial nitric oxide synthase, allowing increased enzyme activity and nitric oxide production in endothelial cells

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